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CMMB - Tutorial 6 Questions with complete solution
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CMMB - Tutorial 6 Questions with complete solution CMMB - Tutorial 6 
 
 
What is the zone of polarizing activity (ZPA)? - correct answer small region in the posterior part of the limb bud 
that acts as a signaling center and mediates anterior-posterior axis formation via limb patterning morphogens 
 
What is a morphogen? - correct answer a protein that defines different cell fates in a concentration-dependent manner. 
 
what does grafting of the ZPA to the anterior region of the limb bud do?...
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CMMB 343 - Final Exam Content Question and answers correctly solved
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CMMB 343 - Final Exam Content Question and answers correctly solved CMMB 343 - Final Exam Content 
 
 
There are as many microbial cells in the human body as there are: - correct answer human cells. 
However, ORFs in the microbiome outnumber ORFs in the human genome 
 
95% of the microbes on humans live in: - correct answer The GI tract (gastrointestinal tract). 
 
Different microhabitats support different... - correct answer ...microbes, depending on temperature, pH, oxygen, etc. 
Each micr...
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CMMB 343 - Lab 2 Question and answers already passed
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CMMB 343 - Lab 2 Question and answers already passed CMMB 343 - Lab 2 
 
 
macromolecules - correct answer amino acids, fatty acids, nucleic acids, carbohydrates 
 
microbial nutrition - correct answer nutrient acquisition, enzymatic transformation, and polymerization of macromolecules 
 
macronutrients - correct answer required in large amounts 
carbon, oxygen, nitrogen, minerals (P, S, K, Mg) 
 
micronutrients - correct answer vitamins (organic growth factors) and minerals (inorganic ions)...
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CMMB 343 - Midterm 2 Question and answers rated A+
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CMMB 343 - Midterm 2 Question and answers rated A+ CMMB 343 - Midterm 2 
 
 
What are 4 DIRECT methods of measuring bacterial growth? - correct answer 1. Flow methods: Coulter counter, flow cytometry 
2. Microscopic counts: Counting chambers 
3. Plate counts 
4. MPNs 
 
Coulter counter - correct answer electronically scans a volume of fluid as it passes through a tiny pipette. The microbes passing through disrupt the electrical current, which is detected. 
 
Flow cytometry - correct answer Si...
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CMMB 343 - Midterm 2 Questions with complete solution
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CMMB 343 - Midterm 2 Questions with complete solution CMMB 343 - Midterm 2 
 
 
Storage compounds - correct answer black dots, 
carbon polymers (glycogen), PHB. PHA. 
Phosphatee granules 
Sulfar gobules 
Minerals 
 
Magnetosomes - correct answer Black circlees ontop of eeachothr 
Magnetic iron allow cell magnetaxis - migrate to magnetic poles 
 
gas vesiclees - correct answer Little diamond shaped things side by side 
bouyancy regulators 
 
Importance of internal membrane - correct answer in...
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CMMB 343 - Midterm Question and answers correctly solved
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CMMB 343 - Midterm Question and answers correctly solved CMMB 343 - Midterm 
 
 
Transformation - correct answer one of three processes that lead to horizontal gene transfer, in which genetic material passes from one bacterium to another 
 
cells able to take up DNA are referred to as - correct answer competent 
 
natural competence - correct answer certain bacteria have specific proteins to take up external DNA 
 
eletroporation - correct answer an electric pulse can use to create temporary...
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CMMB 343 Final Exam Question and answers verified to pass
- Exam (elaborations) • 55 pages • 2024
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CMMB 343 Final Exam Question and answers verified to passCMMB 343 Final Exam 
 
 
How does the microbiome of the human GI tract compare to human genomic cells in the body? (2) 
1. approx 1:1 ratio of human cells to microbes (~10 trillion each) 
 
2. The ORFs in the microbiome outnumber the ORFs in our genome 150:1 
 
 
Define: 
 
1. Microhabitat 
 
2. Microbiota 
 
3. Microbiome 
1. Microhabitat 
 
-location that supports different microbes (skin, GI tract, etc) 
 
-not homologous; skin is a mic...
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CMMB 343 Final Question and answers already passed
- Exam (elaborations) • 62 pages • 2024
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CMMB 343 Final Question and answers already passed CMMB 343 Final 
 
 
How does phi6 penetrate the host cell? - correct answer - because it is an enveloped phage, it uses the entry and uncoating model that relies upon spike-protein mediated endocytosis 
- once in the vesicles, it releases the viral particles and proteases digest the capsid coat, lysing the vesicle, and releasing the phage genome into the cytoplasm 
 
Which protein is the plug at the end of the tail tube in T4, preventing DNA f...
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CMMB 343 Lab Exam 1 Question and answers 100% correct
- Exam (elaborations) • 9 pages • 2024
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CMMB 343 Lab Exam 1 Question and answers 100% correct CMMB 343 Lab Exam 1 
 
 
Ubiquitous - correct answer Organisms are present everywhere unless precautions are made 
 
-Need to be aware of this due to contamination 
 
Aseptic technique - correct answer Sterile technique used to protect from organisms youre working with and to prevent contamination 
 
Microscope - correct answer 
 
How do you get magnification of drawing - correct answer Length of cell/length of the drawing = magnificatio...
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CMMB 343 Lab Midterm Stains Question and answer rated A+
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CMMB 343 Lab Midterm Stains Question and answer rated A+ CMMB 343 Lab Midterm Stains 
 
 
Acid fast (procedure) - correct answer 1. Prepare bacterial smear 
2. Flood smear with Kinyoun carbolfuchsin for 10 min 
3. Rinse slide with water 
4. Decolorize with acid alcohol until runoff clear, rinse water 
5. Counterstain with Brilliant green, rinse water, blot 
 
Acidfast (interpretation) - correct answer positive result= red 
negative result= blue/green 
 
Acid fast - Kinyoun carbolfuschin - cor...